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prehybridization buffer  (Boster Bio)


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    Boster Bio prehybridization buffer
    Prehybridization Buffer, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prehybridization+buffer/pre+hybridization+buffer/pm39463203-42-24-26
    Average 90 stars, based on 1 article reviews
    prehybridization buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Saline:

    Article Title: Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.
    Article Snippet: .. The sections were treated with 0.1% pepsin for 5 min, and then fixed with 4% paraformaldehyde for 5 min. After washing with phosphate-buffered saline, the sections were prehybridized with prehybridization buffer (Boster, Wuhan, China) at 37 C for 2 h. Then the sections were incubated overnight with the digoxigenin (DIG)labeled oligonucleotide probe (5¢-CTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTCGT-3¢, Invitrogen, Shanghai, China) to TK mRNA at 47 C. The probes were visualized with Fab fragments against digoxigenin conjugated with alkaline phosphatase (Roche, Mannheim, Germany) and 5-bromo-4-chloro-3indolyl-phosphate (BCIP)/nitroblue tetrazolium solutions (Roche). ..

    Incubation:

    Article Title: Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.
    Article Snippet: .. The sections were treated with 0.1% pepsin for 5 min, and then fixed with 4% paraformaldehyde for 5 min. After washing with phosphate-buffered saline, the sections were prehybridized with prehybridization buffer (Boster, Wuhan, China) at 37 C for 2 h. Then the sections were incubated overnight with the digoxigenin (DIG)labeled oligonucleotide probe (5¢-CTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTCGT-3¢, Invitrogen, Shanghai, China) to TK mRNA at 47 C. The probes were visualized with Fab fragments against digoxigenin conjugated with alkaline phosphatase (Roche, Mannheim, Germany) and 5-bromo-4-chloro-3indolyl-phosphate (BCIP)/nitroblue tetrazolium solutions (Roche). ..

    Article Title: Diacylglycerol kinase γ facilitates the proliferation and migration of neural stem cells in the developing neural tube.
    Article Snippet: .. Thereafter, the embryos were fixed in 4% PFA containing 0.2% glutaraldehyde for 15 min at 4°C and incubated at 66°C in 1 mL of prehybridization buffer (Boster, Wuhan, China) containing 5 μg of DIG-labeled probe for 24 h. Subsequently, the embryos were incubated with alkaline phosphatase (AP)-conjugated anti-DIG antibody (Roche, Shanghai, China). .. Color reactions were carried out with NBT/BCIP (Roche).

    Article Title: Effect of PTEN antisense oligonucleotide on oesophageal squamous cell carcinoma cell lines.
    Article Snippet: .. After proteinase K treatment, slides were incubated in prehybridization buffer (Boster Biological Technology, Wuhan, China) containing 0.2 N hydrochloric acid, 0.1 M triethanolamine and 0.02 M acetic anhydride for 2 – 4 h at 42 °C. .. Slides were then incubated with 20 μl hybridization buffer (Boster Biological Technology) containing 6 – 12 ng of oligonucleotide probe against PTEN mRNA (5′-CCTCTT GTGCCTTTAAAAATTTGCCC-3′) or MTOR mRNA (5′-CTCAGCGGTAAAAGTGTCCCCTG CCA-3′) (both Beijing AuGCT DNA-SYN Biotechnology) for 12 – 16 h at 42 °C.

    Hybridization:

    Article Title: Long non-coding RNA PVT1 promotes malignancy in human endometrial carcinoma cells through negative regulation of miR-195-5p.
    Article Snippet: .. In brief, slides were treated with 1% paraformaldehyde and blocked with prehybridization buffer (Boster) at 37°C for 4 h. The hybridization mix was prepared with PVT1 probe or miR-195-5p probe in hybridization solution overnight. .. Then, slides was washed with washing buffer (Boster; saline-sodium citrate, SSC).



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